Transforming the untransformable with knockout minicircles: High-efficiency transformation and vector-free allelic exchange knockout in the fish pathogen Photobacterium damselae.

Rudenko, Oleksandra; Baseggio, Laura; McGuigan, Fynn; Barnes, Andrew C

Rudenko, Oleksandra; Baseggio, Laura; McGuigan, Fynn; Barnes, Andrew C.

Afiliação

Rudenko O; School of Biological Sciences and Centre for Marine Science, The University of Queensland, Brisbane, Queensland, Australia.
Baseggio L; School of Biological Sciences and Centre for Marine Science, The University of Queensland, Brisbane, Queensland, Australia.
McGuigan F; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Barnes AC; School of Biological Sciences and Centre for Marine Science, The University of Queensland, Brisbane, Queensland, Australia.

Microbiologyopen ; 12(4): e1374, 2023 08.

Article em En | MEDLINE | ID: mdl-37642481

Assuntos

Eletroporação; Photobacterium; Animais; Photobacterium/genética; Peixes

Palavras-chave

Photobacterium damselae subsp. piscicida; aip56 knockout; high-efficiency transformation; minicircles; vector-free allelic exchange

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Photobacterium / Eletroporação Tipo de estudo: Guideline Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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