Characterization and designing of an SAM riboswitch to establish a high-throughput screening platform for SAM overproduction in Saccharomyces cerevisiae.

Fu, Xiaomeng; Zuo, Xiaoru; Zhao, Xiaomeng; Zhang, Huizhi; Zhang, Chuanbo; Lu, Wenyu

Fu, Xiaomeng; Zuo, Xiaoru; Zhao, Xiaomeng; Zhang, Huizhi; Zhang, Chuanbo; Lu, Wenyu.

Afiliação

Fu X; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zuo X; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zhao X; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zhang H; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zhang C; School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Lu W; Frontiers Science Center for Synthetic Biology, Tianjin University, Tianjin, China.

Biotechnol Bioeng ; 120(12): 3622-3637, 2023 12.

Article em En | MEDLINE | ID: mdl-37691180

Assuntos

Riboswitch; S-Adenosilmetionina; S-Adenosilmetionina/metabolismo; Saccharomyces cerevisiae/genética; Saccharomyces cerevisiae/metabolismo; Ensaios de Triagem em Larga Escala; Riboswitch/genética; Fermentação

Palavras-chave

S-adenosyl- l-methionine; directed evolution; high-throughput screening; metabolic engineering; riboswitch; semirational enzyme design

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: S-Adenosilmetionina / Riboswitch Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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