GM-CSF is a marker of compartmentalised intrathecal inflammation in multiple sclerosis.
Mult Scler
; 29(11-12): 1373-1382, 2023 Oct.
Article
em En
| MEDLINE
| ID: mdl-37700482
ABSTRACT
BACKGROUND:
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a pro-inflammatory cytokine secreted by various immune cells. Several studies have demonstrated an expansion of GM-CSF producing T cells in the blood or CSF of people with MS (pwMS). However, whether this equates to greater concentrations of circulating cytokine remains unknown as quantification is difficult with traditional assays.OBJECTIVE:
To determine whether GM-CSF can be quantified and whether GM-CSF levels are elevated in pwMS.METHODS:
We employed Single Molecule Array (Simoa) to measure GM-CSF in both CSF and blood. We then investigated relationships between GM-CSF levels and measures of blood-CSF-barrier integrity.RESULTS:
GM-CSF was quantifiable in all samples and was significantly higher in the CSF of pwMS compared with controls. No association was found between CSF GM-CSF levels and Q-Albumin - a measure of blood-CSF-barrier integrity. CSF GM-CSF correlated with measures of intrathecal inflammation, and these relationships were greater in primary progressive MS compared with relapsing-remitting MS.CONCLUSION:
GM-CSF levels are elevated specifically in the CSF of pwMS. Our results suggest that elevated cytokine levels may reflect (at least partial) intrathecal production, as opposed to simple diffusion across a dysfunctional blood-CSF-barrier.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Esclerose Múltipla
Limite:
Humans
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article