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Anti-inflammatory protein TSG-6 secreted by BMSCs attenuates silica-induced acute pulmonary inflammation by inhibiting NLRP3 inflammasome signaling in macrophages.
Su, Wenyao; Nong, Qiying; Wu, Jie; Fan, Ruihong; Liang, Yuanting; Hu, Anyi; Gao, Zhongxiang; Liang, Weihui; Deng, Qifei; Wang, Hailan; Xia, Lihua; Huang, Yongshun; Qin, Yiru; Zhao, Na.
Afiliação
  • Su W; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China; Shunde Women and Children's Hospital (Maternity and Child Healthcare Hospital of Shunde Foshan), Guangdong Medical University, Foshan, Guangdong 528300, China; School of Public Health, Guangzhou
  • Nong Q; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China.
  • Wu J; Emeishan Centerfor Disease Control and Prevention, Emeishan, Sichuan 614299, China.
  • Fan R; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China.
  • Liang Y; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China; School of Public Health, Guangzhou Medical University, Guangzhou, Guangdong 511436, China.
  • Hu A; Health Science Center of Shenzhen University, Shenzhen, Guangdong 518060, China.
  • Gao Z; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China.
  • Liang W; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China.
  • Deng Q; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China.
  • Wang H; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China.
  • Xia L; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China.
  • Huang Y; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China.
  • Qin Y; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China. Electronic address: yiru@mail.usf.edu.
  • Zhao N; Guangdong Province Hospital for Occupational Diseases Prevention and Treatment, Guangzhou 510300, China; School of Public Health, Guangzhou Medical University, Guangzhou, Guangdong 511436, China; School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China. Electronic add
Int J Biol Macromol ; 253(Pt 3): 126651, 2023 Dec 31.
Article em En | MEDLINE | ID: mdl-37709227
Silicosis is a severe occupational lung disease caused by inhalation of silica particles. Unfortunately, there are currently limited treatment options available for silicosis. Recent advances have indicated that bone marrow mesenchymal stem cells (BMSCs) have a therapeutic effect on silicosis, but their efficacy and underlying mechanisms remain largely unknown. In this study, we focused on the early phase of silica-induced lung injury to investigate the therapeutic effect of BMSCs. Our findings demonstrated that BMSCs attenuated silica-induced acute pulmonary inflammation by inhibiting NLRP3 inflammasome pathways in lung macrophages. To further understand the mechanisms involved, we utilized RNA sequencing to analyze the transcriptomes of BMSCs co-cultured with silica-stimulated bone marrow-derived macrophages (BMDMs). The results clued tumor necrosis factor-stimulated gene 6 (TSG-6) might be a potentially key paracrine secretion factor released from BMSCs, which exerts a protective effect. Furthermore, the anti-inflammatory and inflammasome pathway inhibition effects of BMSCs were attenuated when TSG-6 expression was silenced, both in vivo and in vitro. Additionally, treatment with exogenous recombinant mouse TSG-6 (rmTSG-6) demonstrated similar effects to BMSCs in attenuating silica-induced inflammation. Overall, our findings suggested that BMSCs can regulate the activation of inflammasome in macrophages by secreting TSG-6, thereby protecting against silica-induced acute pulmonary inflammation both in vivo and in vitro.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia / Silicose / Células-Tronco Mesenquimais Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia / Silicose / Células-Tronco Mesenquimais Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article