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Community-developed checklists for publishing images and image analyses.
Schmied, Christopher; Nelson, Michael S; Avilov, Sergiy; Bakker, Gert-Jan; Bertocchi, Cristina; Bischof, Johanna; Boehm, Ulrike; Brocher, Jan; Carvalho, Mariana T; Chiritescu, Catalin; Christopher, Jana; Cimini, Beth A; Conde-Sousa, Eduardo; Ebner, Michael; Ecker, Rupert; Eliceiri, Kevin; Fernandez-Rodriguez, Julia; Gaudreault, Nathalie; Gelman, Laurent; Grunwald, David; Gu, Tingting; Halidi, Nadia; Hammer, Mathias; Hartley, Matthew; Held, Marie; Jug, Florian; Kapoor, Varun; Koksoy, Ayse Aslihan; Lacoste, Judith; Le Dévédec, Sylvia; Le Guyader, Sylvie; Liu, Penghuan; Martins, Gabriel G; Mathur, Aastha; Miura, Kota; Montero Llopis, Paula; Nitschke, Roland; North, Alison; Parslow, Adam C; Payne-Dwyer, Alex; Plantard, Laure; Ali, Rizwan; Schroth-Diez, Britta; Schütz, Lucas; Scott, Ryan T; Seitz, Arne; Selchow, Olaf; Sharma, Ved P; Spitaler, Martin; Srinivasan, Sathya.
Afiliação
  • Schmied C; Fondazione Human Technopole, Milano, Italy. schmied@fmp-berlin.de.
  • Nelson MS; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany. schmied@fmp-berlin.de.
  • Avilov S; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
  • Bakker GJ; Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.
  • Bertocchi C; Medical BioSciences Department, Radboud University Medical Centre, Nijmegen, the Netherlands.
  • Bischof J; Laboratory for Molecular Mechanics of Cell Adhesions, Pontificia Universidad Católica de Chile Santiago, Santiago de Chile, Chile.
  • Boehm U; Graduate School of Engineering Science, Osaka University, Osaka, Japan.
  • Brocher J; Euro-BioImaging ERIC, Bio-Hub, Heidelberg, Germany.
  • Carvalho MT; Carl Zeiss AG, Oberkochen, Germany.
  • Chiritescu C; Scientific Image Processing and Analysis, BioVoxxel, Ludwigshafen, Germany.
  • Christopher J; Nanophotonics and BioImaging Facility at INL, International Iberian Nanotechnology Laboratory, Braga, Portugal.
  • Cimini BA; Phi Optics, Inc., Champaign, IL, USA.
  • Conde-Sousa E; Biochemistry Center Heidelberg, Heidelberg University, Heidelberg, Germany.
  • Ebner M; Imaging Platform, Broad Institute, Cambridge, MA, USA.
  • Ecker R; i3S, Instituto de Investigação e Inovação Em Saúde and INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.
  • Eliceiri K; Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
  • Fernandez-Rodriguez J; Translational Research Institute, Queensland University of Technology, Woolloongabba, Queensland, Australia.
  • Gaudreault N; School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia.
  • Gelman L; TissueGnostics GmbH, Vienna, Austria.
  • Grunwald D; Department of Medical Physics and Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.
  • Gu T; Centre for Cellular Imaging Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
  • Halidi N; Allen Institute for Cell Science, Seattle, WA, USA.
  • Hammer M; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
  • Hartley M; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.
  • Held M; University of Oklahoma, Norman, OK, USA.
  • Jug F; Advanced Light Microscopy Unit, Centre for Genomic Regulation, Barcelona, Spain.
  • Kapoor V; RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.
  • Koksoy AA; European Molecular Biology Laboratory (EMBL), European Bioinformatics Institute, Hinxton, UK.
  • Lacoste J; Centre for Cell Imaging, the University of Liverpool, Liverpool, UK.
  • Le Dévédec S; Fondazione Human Technopole, Milano, Italy.
  • Le Guyader S; Department of AI Research, Kapoor Labs, Paris, France.
  • Liu P; MD Anderson Cancer Center, Houston, TX, USA.
  • Martins GG; MIA Cellavie Inc., Montreal, Quebec, Canada.
  • Mathur A; Division of Drug Discovery and Safety, Cell Observatory, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands.
  • Miura K; Karolinska Institutet, Huddinge, Sweden.
  • Montero Llopis P; Key Laboratory for Modern Measurement Technology and Instruments of Zhejiang Province, College of Optical and Electronic Technology, China Jiliang University, Hangzhou, China.
  • Nitschke R; Advanced Imaging Facility, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
  • North A; Euro-BioImaging ERIC, Bio-Hub, Heidelberg, Germany.
  • Parslow AC; Bioimage Analysis and Research, Heidelberg, Germany.
  • Payne-Dwyer A; MicRoN Core, Harvard Medical School, Boston, MA, USA.
  • Plantard L; Life Imaging Center, Signalling Research Centres CIBSS and BIOSS, University of Freiburg, Freiburg, Germany.
  • Ali R; Bio-Imaging Resource Center, the Rockefeller University, New York, NY, USA.
  • Schroth-Diez B; Baker Institute Microscopy Platform, Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia.
  • Schütz L; School of Physics, Engineering and Technology, University of York, Heslington, UK.
  • Scott RT; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
  • Seitz A; King Abdullah International Medical Research Center (KAIMRC), Medical Research Core Facility and Platforms (MRCFP), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.
  • Selchow O; Light Microscopy Facility, Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Dresden, Germany.
  • Sharma VP; ariadne.ai (Germany) GmbH, Heidelberg, Germany.
  • Spitaler M; Space Biosciences Division, NASA Ames Research Center, Moffett Field, CA, USA.
  • Srinivasan S; BioImaging and Optics Platform, Faculty of Life Sciences (SV), École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Nat Methods ; 21(2): 170-181, 2024 Feb.
Article em En | MEDLINE | ID: mdl-37710020
ABSTRACT
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Editoração / Lista de Checagem Tipo de estudo: Guideline Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Editoração / Lista de Checagem Tipo de estudo: Guideline Idioma: En Ano de publicação: 2024 Tipo de documento: Article