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Vascular adhesion protein-1-targeted PET imaging in autoimmune myocarditis.
Jahandideh, Arghavan; Virta, Jenni; Li, Xiang-Guo; Liljenbäck, Heidi; Moisio, Olli; Ponkamo, Jesse; Rajala, Noora; Alix, Marion; Lehtonen, Jukka; Mäyränpää, Mikko I; Salminen, Tiina A; Knuuti, Juhani; Jalkanen, Sirpa; Saraste, Antti; Roivainen, Anne.
Afiliação
  • Jahandideh A; Turku PET Centre, University of Turku, Åbo Akademi University and Turku University Hospital, 20520, Turku, Finland.
  • Virta J; Turku PET Centre, University of Turku, Åbo Akademi University and Turku University Hospital, 20520, Turku, Finland.
  • Li XG; Turku PET Centre, University of Turku, Åbo Akademi University and Turku University Hospital, 20520, Turku, Finland.
  • Liljenbäck H; InFLAMES Research Flagship Center, University of Turku, Turku, Finland.
  • Moisio O; Department of Chemistry, University of Turku, Turku, Finland.
  • Ponkamo J; Turku PET Centre, University of Turku, Åbo Akademi University and Turku University Hospital, 20520, Turku, Finland.
  • Rajala N; Turku Center for Disease Modeling, University of Turku, Turku, Finland.
  • Alix M; Turku PET Centre, University of Turku, Åbo Akademi University and Turku University Hospital, 20520, Turku, Finland.
  • Lehtonen J; Turku PET Centre, University of Turku, Åbo Akademi University and Turku University Hospital, 20520, Turku, Finland.
  • Mäyränpää MI; Turku PET Centre, University of Turku, Åbo Akademi University and Turku University Hospital, 20520, Turku, Finland.
  • Salminen TA; Structural Bioinformatics Laboratory, Åbo Akademi University, Turku, Finland.
  • Knuuti J; Heart and Lung Center, Helsinki University and Helsinki University Hospital, Helsinki, Finland.
  • Jalkanen S; Department of Pathology, Helsinki University and Helsinki University Hospital, Helsinki, Finland.
  • Saraste A; InFLAMES Research Flagship Center, University of Turku, Turku, Finland.
  • Roivainen A; Structural Bioinformatics Laboratory, Åbo Akademi University, Turku, Finland.
J Nucl Cardiol ; 30(6): 2760-2772, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37758963
BACKGROUND: Vascular adhesion protein-1 (VAP-1) is an adhesion molecule and primary amine oxidase, and Gallium-68-labeled 1,4,7,10-tetraazacyclododecane-N,N',N″,N‴-tetra-acetic acid conjugated sialic acid-binding immunoglobulin-like lectin 9 motif containing peptide ([68Ga]Ga-DOTA-Siglec-9) is a positron emission tomography (PET) tracer targeting VAP-1. We evaluated the feasibility of PET imaging with [68Ga]Ga-DOTA-Siglec-9 for the detection of myocardial lesions in rats with autoimmune myocarditis. METHODS: Rats (n = 9) were immunized twice with porcine cardiac myosin in complete Freund's adjuvant. Control rats (n = 6) were injected with Freund's adjuvant alone. On day 21, in vivo PET/computed tomography (CT) imaging with [68Ga]Ga-DOTA-Siglec-9 was performed, followed by ex vivo autoradiography, histology, and immunohistochemistry of tissue sections. In addition, myocardial samples from three patients with cardiac sarcoidosis were studied. RESULTS: [68Ga]Ga-DOTA-Siglec-9 PET/CT images of immunized rats showed higher uptake in myocardial lesions than in myocardium outside lesions (SUVmean, 0.5 ± 0.1 vs 0.3 ± 0.1; P = .003) or control rats (SUVmean, 0.2 ± 0.03; P < .0001), which was confirmed by ex vivo autoradiography of tissue sections. Immunohistochemistry showed VAP-1-positive staining in lesions of rats with myocarditis and in patients with cardiac sarcoidosis. CONCLUSION: VAP-1-targeted [68Ga]Ga-DOTA-Siglec-9 PET is a potential novel technique for the detection of myocardial lesions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sarcoidose / Miocardite Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sarcoidose / Miocardite Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article