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A simplified bacterial community found within the epidermis than at the epidermal surface of atopic dermatitis patients and healthy controls.
Barnes, Christopher J; Asplund, Maria; Clausen, Maja-Lisa; Rasmussen, Linett; Olesen, Caroline Meyer; Yüksel, Yasemin Topal; Andersen, Paal Skytt; Litman, Thomas; Holmstrøm, Kim; Bay, Lene; Fritz, Blaine Gabriel; Bjarnsholt, Thomas; Agner, Tove; Hansen, Anders Johannes.
Afiliação
  • Barnes CJ; The Globe Institute, Faculty of Health, University of Copenhagen, Copenhagen K, 1350, Denmark. drcjbarnes@gmail.com.
  • Asplund M; Department of Agroecology, Faculty of Technical Sciences, Aarhus University, Forsøgsvej 1, Slagelse, 4200, Denmark. drcjbarnes@gmail.com.
  • Clausen ML; The Globe Institute, Faculty of Health, University of Copenhagen, Copenhagen K, 1350, Denmark.
  • Rasmussen L; Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark.
  • Olesen CM; The Globe Institute, Faculty of Health, University of Copenhagen, Copenhagen K, 1350, Denmark.
  • Yüksel YT; Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark.
  • Andersen PS; Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark.
  • Litman T; Department of Bacteria, Parasites and Fungi, Statens Serum Insitute, Copenhagen, Denmark.
  • Holmstrøm K; Department of Immunology and Microbiology, LEO Foundation Skin Immunology Research Center, University of Copenhagen, Copenhagen, Denmark.
  • Bay L; Explorative Biology and Bioinformatics, LEO Pharma A/S, Ballerup, Denmark.
  • Fritz BG; Bioneer A/S, Kogle Allé 2, Hørsholm, Denmark.
  • Bjarnsholt T; Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
  • Agner T; Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
  • Hansen AJ; Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
BMC Microbiol ; 23(1): 273, 2023 09 29.
Article em En | MEDLINE | ID: mdl-37773096
There has been considerable research into the understanding of the healthy skin microbiome. Similarly, there is also a considerable body of research into whether specific microbes contribute to skin disorders, with atopic dermatitis (AD) routinely linked to increased Staphylococcus aureus (S. aureus) colonisation. In this study, the epidermal surface of participants was sampled using swabs, while serial tape-stripping (35 tapes) was performed to sample through the stratum corneum. Samples were taken from AD patients and healthy controls, and the bacterial communities were profiled by metabarcoding the universal V3-V4 16S rRNA region. Results show that the majority of bacterial richness is located within the outermost layers of the stratum corneum, however there were many taxa that were found almost exclusively at the very outermost layer of the epidermis. We therefore hypothesise that tape-stripping can be performed to investigate the 'core microbiome' of participants by removing environmental contaminants. Interestingly, significant community variation between AD patients and healthy controls was only observable at the epidermal surface, yet a number of individual taxa were found to consistently differ with AD status across the entire epidermis (i.e. both the epidermal surface and within the epidermis). Sampling strategy could therefore be tailored dependent on the hypothesis, with sampling for forensic applications best performed using surface swabs and outer tapes, while profiling sub-surface communities may better reflect host genome and immunological status.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dermatite Atópica Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dermatite Atópica Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article