Determining superoxide dismutase content and catalase activity in mammalian cell lines.
MethodsX
; 11: 102395, 2023 Dec.
Article
em En
| MEDLINE
| ID: mdl-37791011
ABSTRACT
Traditional methods for determining superoxide dismutase (SOD) content and catalase (CAT) activity rely on measuring the absorbance of individual tissue (biological) samples using a cuvette and spectrophotometer, rather than cell cultures. Although there are kits available for SOD and CAT assays, these allow for high-throughput analysis of samples and might be too expensive for research laboratories in countries from the Global South, such as South Africa. This paper describes a simple and cost-effective method to determine SOD content and CAT activity in mammalian cell cultures following exposure to environmental chemical mixtures by measuring absorbance in 96-well microplates. Moreover, the equipment used for this method is considered standard for cell culture laboratories, while the reagents and consumables are easily obtainable.â¢Antioxidant enzyme levels can be measured in vitro in cell cultures.â¢The supernatant obtained can be used to determine protein concentration, SOD content, and CAT activity.â¢This method is simple and affordable, allowing for the analysis of multiple samples (up to 32 samples per microplate).
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article