The adaptability of the ion-binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF.
J Biol Chem
; 299(11): 105331, 2023 Nov.
Article
em En
| MEDLINE
| ID: mdl-37820867
ABSTRACT
The periplasmic chaperone SilF has been identified as part of an Ag(I) detoxification system in Gram-negative bacteria. Sil proteins also bind Cu(I) but with reported weaker affinity, therefore leading to the designation of a specific detoxification system for Ag(I). Using isothermal titration calorimetry, we show that binding of both ions is not only tighter than previously thought but of very similar affinities. We investigated the structural origins of ion binding using molecular dynamics and QM/MM simulations underpinned by structural and biophysical experiments. The results of this analysis showed that the binding site adapts to accommodate either ion, with key interactions with the solvent in the case of Cu(I). The implications of this are that Gram-negative bacteria do not appear to have evolved a specific Ag(I) efflux system but take advantage of the existing Cu(I) detoxification system. Therefore, there are consequences for how we define a particular metal resistance mechanism and understand its evolution in the environment.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cobre
/
Escherichia coli
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article