Noncovalent Tagging for Identifying Unknown Contaminants of Specific Bioactivity in Environmental Water.
Anal Chem
; 95(43): 15851-15855, 2023 10 31.
Article
em En
| MEDLINE
| ID: mdl-37857307
ABSTRACT
Identifying contaminants of specific bioactivities from complicated environmental matrices remains costly and time-consuming, as it requires us to not only resolve their structures but also determine their bioactivities. Herein, a novel noncovalent tagging method is integrated in mass spectrometry for identifying unknown contaminants that target dopamine (DA) receptors. Via proteolysis of bovine serum albumin, a stereoselective hexapeptide (ACFAVE) is selected for noncovalently tagging the contaminants that possess the stereostructural characteristics of binding to DA receptors. The tagged contaminants can be readily distinguished from the coexisting species for subsequent structural analysis based on the tagging-induced shifts of the mass-to-charge ratios. Thus, both bioactivity evaluation and structure analysis are accomplished via mass spectrometry. By using this method, 1,3-diphenylguanidine (DPG), a widely used additive in rubber and plastics, is successfully identified out of 2495 features detected in the Pearl River water, with its concentration determined as only 9.8 µg L-1. Furthermore, DPG is confirmed as a potential disrupter to the DA receptors via a simulated docking experiment, which has not been reported before. The present noncovalent tagging method provides a cost-effective and time-efficient way of identifying bioactive molecules in complicated matrices. And proteolysis of proteins is promising for developing more taggants with other desired stereoselectivities in the future.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
/
Água
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article