Your browser doesn't support javascript.
loading
The GPCR adaptor protein Norbin regulates S1PR1 trafficking and the morphology, cell cycle and survival of PC12 cells.
Johansen, Valdemar B I; Hampson, Elizabeth; Tsonou, Elpida; Pantarelli, Chiara; Chu, Julia Y; Crossland, Laraine; Okkenhaug, Hanneke; Massey, Andrew J; Hornigold, David C; Welch, Heidi C E; Chetwynd, Stephen A.
Afiliação
  • Johansen VBI; Signalling Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK.
  • Hampson E; Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.
  • Tsonou E; Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
  • Pantarelli C; Signalling Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK.
  • Chu JY; Vernalis (R&D) Ltd., Cambridge, UK.
  • Crossland L; Signalling Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK.
  • Okkenhaug H; Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Cambridge, UK.
  • Massey AJ; Signalling Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK.
  • Hornigold DC; Signalling Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK.
  • Welch HCE; Signalling Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK.
  • Chetwynd SA; Imaging Facility, The Babraham Institute, Cambridge, UK.
Sci Rep ; 13(1): 18237, 2023 10 25.
Article em En | MEDLINE | ID: mdl-37880240
Norbin is an adaptor protein that binds numerous G protein-coupled receptors (GPCRs), is highly expressed in neurons, and is essential for a functioning nervous system in rodent models. Yet, beyond its control of neurite outgrowth and synaptic plasticity, few cellular roles of Norbin have been investigated to date. Furthermore, while Norbin is known to regulate the steady-state cell surface levels of several GPCRs, only in one case has the protein been shown to control the agonist-induced receptor internalisation which serves to attenuate GPCR signalling. Here, we generated a Norbin-deficient PC12 cell line which enabled us to study both the cellular functions of Norbin and its roles in GPCR trafficking and signalling. We show that Norbin limits cell size and spreading, and is required for the growth, viability and cell cycle progression of PC12 cells. We also found that Norbin regulates both the steady-state surface level and agonist-induced internalisation of the GPCR sphingosine-1-phosphate receptor 1 (S1PR1) in these cells, suggesting that its role in agonist-dependent GPCR trafficking is more widespread than previously appreciated. Finally, we show that Norbin limits the S1P-stimulated activation of Akt and p38 Mapk, and is required for the activation of Erk in PC12 cells. Together, our findings provide a better understanding of the cellular functions of Norbin and its control of GPCR trafficking.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Receptores Acoplados a Proteínas G Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Receptores Acoplados a Proteínas G Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article