Deep mutational scanning reveals the functional constraints and evolutionary potential of the influenza A virus PB1 protein.
J Virol
; 97(11): e0132923, 2023 Nov 30.
Article
em En
| MEDLINE
| ID: mdl-37882522
ABSTRACT
IMPORTANCE The influenza virus polymerase is important for adaptation to new hosts and, as a determinant of mutation rate, for the process of adaptation itself. We performed a deep mutational scan of the polymerase basic 1 (PB1) protein to gain insights into the structural and functional constraints on the influenza RNA-dependent RNA polymerase. We find that PB1 is highly constrained at specific sites that are only moderately predicted by the global structure or larger domain. We identified a number of beneficial mutations, many of which have been shown to be functionally important or observed in influenza virus' natural evolution. Overall, our atlas of PB1 mutations and their fitness impacts serves as an important resource for future studies of influenza replication and evolution.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
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Proteínas Virais
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RNA Polimerase Dependente de RNA
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Mutação
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article