Processing of boar spermatozoa with phosphate-buffered saline at 4ËC induces an increase in 32 kDa tyrosine-phosphorylated protein (p32).
Vet Res Commun
; 48(2): 1189-1193, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-37889425
ABSTRACT
We aimed to investigate the impact of processing boar spermatozoa with phosphate-buffered saline (PBS) at 4 ËC on acrosomal integrity and increase in 32 kDa tyrosine-phosphorylated protein (p32). Following cooled PBS washing, we observed a significant increase in p32 levels and in the proportion of dead spermatozoa with compromised acrosomal integrity compared to sperm washing using PBS at room temperature. Interestingly, this increase in p32 was effectively inhibited when cooled PBS was supplemented with 1 mM AEBSF, a serine protease inhibitor. Our findings suggest that the increase of p32 in response to cooled PBS washing in boar spermatozoa is associated with enhanced protease activity in dead spermatozoa.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatos
/
Espermatozoides
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article