Perturbations in lipid metabolism and gut microbiota composition precede cardiac dysfunction in a mouse model of thalassemia.

ABSTRACT

Cardiomyopathy is a major complication of thalassemia, yet the precise underlying molecular mechanisms remain unclear. We examined whether altered lipid metabolism is an early driving factor in the development of cardiomyopathy using the Th3/+ mouse model of thalassemia. At age 20 weeks, male and female Th3/+ mice manifested anemia and iron overload; however, only males displayed metabolic defects and altered cardiac function. Untargeted lipidomics indicated that the circulating levels of 35 lipid species were significantly altered in Th3/+ mice compared to wild-type controls triglycerides (TGs) with saturated fatty acids (FAs; TG420 and TG440) were elevated, while TGs with unsaturated FAs (TG(182_205_182 and TG548)) were reduced. Similarly, phosphatidylcholines (PCs) with long chain FAs (palmitic (160) or oleic (181)) were increased, while PCs with polyunsaturated FAs decreased. Circulating PC(160_140), GlcCer(d181/240) correlated significantly with iron overload and cardiac hypertrophy. 16S rRNA gene profiling revealed alterations in the intestinal microbiota of Th3/+ mice. Differentially abundant bacterial genera correlated with PC(396), PC(181_226), GlcCer(d181/241) and CE(140). These results provide new knowledge on perturbations in lipid metabolism and the gut microbiota of Th3/+ mice and identify specific factors which may represent early biomarkers or therapeutic targets to prevent development of cardiomyopathy in ß-thalassemia.