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The impact of ORF19.36.1 in the pathobiology of Candida albicans.
Arita, Glaucia Sayuri; Ma, Qinxi; Leaves, Ian; Pradhan, Arnab; Hickey, Emer; Dambuza, Ivy; Bebes, Attila; Vincenzi Conrado, Pollyanna Cristina; Barros Galinari, Camila; Vicente Seixas, Flávio Augusto; Kioshima, Érika Seki; de Souza Bonfim-Mendonça, Patrícia; Svidzinski, Terezinha Inez Estivalet; Brown, Alistair J P.
Afiliação
  • Arita GS; Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringa, T20 Building, Room 203, Colombo Avenue, 5790 - Zone 7, Maringá, PR, 87020-900, Brazil.
  • Ma Q; Medical Research Council Centre for Medical Mycology, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK.
  • Leaves I; Medical Research Council Centre for Medical Mycology, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK.
  • Pradhan A; Medical Research Council Centre for Medical Mycology, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK.
  • Hickey E; Medical Research Council Centre for Medical Mycology, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK.
  • Dambuza I; Medical Research Council Centre for Medical Mycology, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK.
  • Bebes A; Centre for Cytomics, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK.
  • Vincenzi Conrado PC; Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringa, T20 Building, Room 203, Colombo Avenue, 5790 - Zone 7, Maringá, PR, 87020-900, Brazil.
  • Barros Galinari C; Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringa, T20 Building, Room 203, Colombo Avenue, 5790 - Zone 7, Maringá, PR, 87020-900, Brazil.
  • Vicente Seixas FA; Department of Technology, State University of Maringa, Ângelo Moreira da Fonseca Avenue, 1800 - Danielle Park, Umuarama, PR, 87506-370, Brazil.
  • Kioshima ÉS; Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringa, T20 Building, Room 203, Colombo Avenue, 5790 - Zone 7, Maringá, PR, 87020-900, Brazil.
  • de Souza Bonfim-Mendonça P; Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringa, T20 Building, Room 203, Colombo Avenue, 5790 - Zone 7, Maringá, PR, 87020-900, Brazil.
  • Svidzinski TIE; Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringa, T20 Building, Room 203, Colombo Avenue, 5790 - Zone 7, Maringá, PR, 87020-900, Brazil. Electronic address: tiesvidzinski@uem.br.
  • Brown AJP; Medical Research Council Centre for Medical Mycology, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK.
Microb Pathog ; 185: 106437, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37913825
ABSTRACT

BACKGROUND:

Our previous proteomics data obtained from Candida albicans recovered after serial passage in a murine model of systemic infection revealed that Orf19.36.1 expression correlates with the virulence of the fungus. Therefore, the impact of ORF19.36.1 upon virulence was tested in this study. MATERIALS &

METHODS:

CRISPR-Cas9 technology was used to construct homozygous C. albicans orf19.36.1 null mutants and the phenotypes of these mutants examined in vitro (filamentation, invasion, adhesion, biofilm formation, hydrolase activities) and in vivo assays.

RESULTS:

The deletion of ORF19.36.1 did not significantly impact the phenotypes examined or the virulence of C. albicans in two infection models.

CONCLUSION:

These results suggest that, although Orf19.36.1 expression correlates with virulence, this protein is not essential for C. albicans pathobiology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida albicans / Candidíase / Proteínas Fúngicas Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida albicans / Candidíase / Proteínas Fúngicas Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article