The impact of ORF19.36.1 in the pathobiology of Candida albicans.
Microb Pathog
; 185: 106437, 2023 Dec.
Article
em En
| MEDLINE
| ID: mdl-37913825
ABSTRACT
BACKGROUND:
Our previous proteomics data obtained from Candida albicans recovered after serial passage in a murine model of systemic infection revealed that Orf19.36.1 expression correlates with the virulence of the fungus. Therefore, the impact of ORF19.36.1 upon virulence was tested in this study. MATERIALS &METHODS:
CRISPR-Cas9 technology was used to construct homozygous C. albicans orf19.36.1 null mutants and the phenotypes of these mutants examined in vitro (filamentation, invasion, adhesion, biofilm formation, hydrolase activities) and in vivo assays.RESULTS:
The deletion of ORF19.36.1 did not significantly impact the phenotypes examined or the virulence of C. albicans in two infection models.CONCLUSION:
These results suggest that, although Orf19.36.1 expression correlates with virulence, this protein is not essential for C. albicans pathobiology.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Candida albicans
/
Candidíase
/
Proteínas Fúngicas
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article