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Electrolyzed hypochlorous acid water exhibits potent disinfectant activity against various viruses through irreversible protein aggregation.
Dianty, Rahmi; Hirano, Junki; Anzai, Itsuki; Kanai, Yuta; Hayashi, Tsuyoshi; Morimoto, Masae; Kataoka-Nakamura, Chikako; Kobayashi, Sakura; Uemura, Kentaro; Ono, Chikako; Watanabe, Tokiko; Kobayashi, Takeshi; Murakami, Kosuke; Kikuchi, Kenji; Hotta, Kunimoto; Yoshikawa, Toshikazu; Taguwa, Shuhei; Matsuura, Yoshiharu.
Afiliação
  • Dianty R; Laboratory of Virus Control, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
  • Hirano J; Laboratory of Virus Control, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Anzai I; Laboratory of Virus Control, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
  • Kanai Y; Laboratory of Virus Control, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Hayashi T; Laboratory of Virus Control, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
  • Morimoto M; Laboratory of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Kataoka-Nakamura C; Laboratory of Virus Control, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
  • Kobayashi S; Laboratory of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Uemura K; Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
  • Ono C; Innovative Vaccine Research and Development Center, The Research Foundation for Microbial Diseases of Osaka University, Osaka, Japan.
  • Watanabe T; Innovative Vaccine Research and Development Center, The Research Foundation for Microbial Diseases of Osaka University, Osaka, Japan.
  • Kobayashi T; Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
  • Murakami K; Laboratory of Virus Control, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
  • Kikuchi K; Laboratory of Virus Control, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Hotta K; Laboratory of Virus Control, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
  • Yoshikawa T; Laboratory of Virus Control, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Taguwa S; Laboratory of Virus Control, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
  • Matsuura Y; Laboratory of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Front Microbiol ; 14: 1284274, 2023.
Article em En | MEDLINE | ID: mdl-37928667
ABSTRACT
It is essential to employ efficient measures to prevent the transmission of pathogenic agents during a pandemic. One such method involves using hypochlorous acid (HClO) solution. The oxidative properties of HClO water (HAW) can contribute to its ability to eliminate viral particles. Here, we examined a highly purified slightly acidic hypochlorous acid water (Hp-SA-HAW) obtained from the reverse osmosis membrane treatment of an electrolytically-generated SA-HAW for its anti-viral activity and mode of action on viral proteins. Hp-SA-HAW exhibited broad-spectrum antiviral effects against various viruses, including adenovirus, hepatitis B virus, Japanese encephalitis virus (JEV), and rotavirus. Additionally, Hp-SA-HAW treatment dose-dependently resulted in irreversibly aggregated multimers of the JEV envelope and capsid proteins. However, Hp-SA-HAW treatment had no discernible effect on viral RNA, indicating that Hp-SA-HAW acts against amino acids rather than nucleic acids. Furthermore, Hp-SA-HAW substantially reduced the infectivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including the ancestral variant and other multiple variants. Hp-SA-HAW treatment induced the aggregation of the SARS-CoV-2 spike and nuclear proteins and disrupted the binding of the purified spike protein of SARS-CoV-2 to human ACE2. This study demonstrates that the broad-spectrum virucidal activity of highly purified HClO is attributed to viral protein aggregation of virion via protein oxidation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article