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Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection.
Helble, Jennifer D; Walsh, Michael J; McCarthy, Julie E; Smith, Neal P; Tirard, Alice J; Arnold, Benjamin Y; Villani, Alexandra-Chloe; Hu, Linden T.
Afiliação
  • Helble JD; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
  • Walsh MJ; Division of Gastroenterology, Massachusetts General Hospital, Boston, MA 02114, USA.
  • McCarthy JE; Department of Cancer Immunology and Virology, Dana Farber Cancer Institute, Boston, MA 02215, USA.
  • Smith NP; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
  • Tirard AJ; Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.
  • Arnold BY; Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.
  • Villani AC; Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.
  • Hu LT; Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.
iScience ; 26(11): 108217, 2023 Nov 17.
Article em En | MEDLINE | ID: mdl-37953958
Lyme disease is caused by the bacterial pathogen Borrelia burgdorferi, which can be readily modeled in laboratory mice. In order to understand the cellular and transcriptional changes that occur during B. burgdorferi infection, we conducted single-cell RNA sequencing (scRNA-seq) of ankle joints of infected C57BL/6 mice over time. We found that macrophages/monocytes, T cells, synoviocytes and fibroblasts all showed significant differences in gene expression of both inflammatory and non-inflammatory genes that peaked early and returned to baseline before the typical resolution of arthritis. Predictions of cellular interactions showed that macrophages appear to communicate extensively between different clusters of macrophages as well as with fibroblasts and synoviocytes. Our data give unique insights into the interactions between B. burgdorferi and the murine immune system over time and allow for a better understanding of mechanisms by which the dysregulation of the immune response may lead to prolonged symptoms in some patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article