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Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes.
Ren, Ren; Cai, Shenglin; Fang, Xiaona; Wang, Xiaoyi; Zhang, Zheng; Damiani, Micol; Hudlerova, Charlotte; Rosa, Annachiara; Hope, Joshua; Cook, Nicola J; Gorelkin, Peter; Erofeev, Alexander; Novak, Pavel; Badhan, Anjna; Crone, Michael; Freemont, Paul; Taylor, Graham P; Tang, Longhua; Edwards, Christopher; Shevchuk, Andrew; Cherepanov, Peter; Luo, Zhaofeng; Tan, Weihong; Korchev, Yuri; Ivanov, Aleksandar P; Edel, Joshua B.
Afiliação
  • Ren R; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.
  • Cai S; Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK.
  • Fang X; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London, W12 0BZ, UK. shenglin.cai15@imperial.ac.uk.
  • Wang X; Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. shenglin.cai15@imperial.ac.uk.
  • Zhang Z; The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Aptamer Selection Center, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, 310022, Hangzhou, Zhejiang, China.
  • Damiani M; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.
  • Hudlerova C; The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Aptamer Selection Center, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, 310022, Hangzhou, Zhejiang, China.
  • Rosa A; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.
  • Hope J; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, 82 Wood Lane, London, W12 0BZ, UK.
  • Cook NJ; The Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.
  • Gorelkin P; Wolfson Education Centre, Faculty of Medicine, Imperial College London, London, UK.
  • Erofeev A; The Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.
  • Novak P; The Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.
  • Badhan A; National University of Science and Technology "MISIS", Leninskiy Prospect 4, 119991, Moscow, Russian Federation.
  • Crone M; National University of Science and Technology "MISIS", Leninskiy Prospect 4, 119991, Moscow, Russian Federation.
  • Freemont P; ICAPPIC Limited, The Fisheries, Mentmore Terrace, London, E8 3PN, UK.
  • Taylor GP; Molecular Diagnostic Unit, Section of Virology, Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
  • Tang L; Section of Structural and Synthetic Biology, Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
  • Edwards C; Section of Structural and Synthetic Biology, Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
  • Shevchuk A; Molecular Diagnostic Unit, Section of Virology, Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
  • Cherepanov P; State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, 310027, Hangzhou, China.
  • Luo Z; Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK.
  • Tan W; ICAPPIC Limited, The Fisheries, Mentmore Terrace, London, E8 3PN, UK.
  • Korchev Y; Department of Metabolism, Digestion and Reproduction, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK.
  • Ivanov AP; The Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, UK.
  • Edel JB; Molecular Diagnostic Unit, Section of Virology, Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK.
Nat Commun ; 14(1): 7362, 2023 11 14.
Article em En | MEDLINE | ID: mdl-37963924
ABSTRACT
We report on single-molecule nanopore sensing combined with position-encoded DNA molecular probes, with chemistry tuned to simultaneously identify various antigen proteins and multiple RNA gene fragments of SARS-CoV-2 with high sensitivity and selectivity. We show that this sensing strategy can directly detect spike (S) and nucleocapsid (N) proteins in unprocessed human saliva. Moreover, our approach enables the identification of RNA fragments from patient samples using nasal/throat swabs, enabling the identification of critical mutations such as D614G, G446S, or Y144del among viral variants. In particular, it can detect and discriminate between SARS-CoV-2 lineages of wild-type B.1.1.7 (Alpha), B.1.617.2 (Delta), and B.1.1.539 (Omicron) within a single measurement without the need for nucleic acid sequencing. The sensing strategy of the molecular probes is easily adaptable to other viral targets and diseases and can be expanded depending on the application required.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanoporos / Antígenos Virais Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanoporos / Antígenos Virais Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article