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Free PCR virus detection via few-layer bismuthene and tetrahedral DNA nanostructured assemblies.
Gutiérrez-Gálvez, Laura; García-Fernández, Daniel; Barrio, Melisa Del; Luna, Mónica; Torres, Íñigo; Zamora, Félix; Navío, Cristina; Milán-Rois, Paula; Castellanos, Milagros; Abreu, Melanie; Cantón, Rafael; Galán, Juan Carlos; Somoza, Álvaro; Miranda, Rodolfo; García-Mendiola, Tania; Lorenzo, Encarnación.
Afiliação
  • Gutiérrez-Gálvez L; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid (UAM), 28049, Madrid, Spain.
  • García-Fernández D; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid (UAM), 28049, Madrid, Spain.
  • Barrio MD; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid (UAM), 28049, Madrid, Spain.
  • Luna M; Instituto de Micro y Nanotecnología IMN-CNM, CSIC (CEI UAM+CSIC), 28760, Tres Cantos, Madrid, Spain.
  • Torres Í; Departamento de Química Inorgánica and Condensed Matter Physics Center (IFIMAC), Universidad Autónoma de Madrid, 28049, Madrid, Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain.
  • Zamora F; Departamento de Química Inorgánica and Condensed Matter Physics Center (IFIMAC), Universidad Autónoma de Madrid, 28049, Madrid, Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain.
  • Navío C; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain.
  • Milán-Rois P; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain.
  • Castellanos M; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain.
  • Abreu M; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034, Madrid, Spain.
  • Cantón R; CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.
  • Galán JC; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034, Madrid, Spain; Centro de Investigación Biomédica en Red en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
  • Somoza Á; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain.
  • Miranda R; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049, Madrid, Spain.
  • García-Mendiola T; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid (UAM), 28049, Madrid, Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain. Electronic address: tania.garcia@uam.es.
  • Lorenzo E; Departamento de Química Analítica y Análisis Instrumental, Universidad Autónoma de Madrid (UAM), 28049, Madrid, Spain; Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain; IMDEA-Nanociencia, Ciudad Universitaria de Cantoblanco, 28049,
Talanta ; 269: 125405, 2024 Mar 01.
Article em En | MEDLINE | ID: mdl-37984235
In this work we describe a highly sensitive method based on a biocatalyzed electrochemiluminescence approach. The system combines, for the first time, the use of few-layer bismuthene (FLB) as a platform for the oriented immobilization of tetrahedral DNA nanostructures (TDNs) specifically designed and synthetized to detect a specific SARS-CoV-2 gene sequence. In one of its vertices, these TDNs contain a DNA capture probe of the open reading frame 1 ab (ORF1ab) of the virus, available for the biorecognition of the target DNA/RNA. At the other three vertices, there are thiol groups that enable the stable anchoring/binding to the FLB surface. This novel geometry/approach enables not only the binding of the TDNs to surfaces, but also the orientation of the capture probe in a direction normal to the bismuthine surface so that it is readily accessible for binding/recognition of the specific SARS-CoV-2 sequence. The analytical signal is based on the anodic electrochemiluminescence (ECL) intensity of luminol which, in turn, arises as a result of the reaction with H2O2, generated by the enzymatic reaction of glucose oxidation, catalyzed by the biocatalytic label avidin-glucose oxidase conjugate (Av-GOx), which acts as co-reactant in the electrochemiluminescent reaction. The method exhibits a limit of detection (LOD) of 4.31 aM and a wide linear range from 14.4 aM to 1.00 µM, and its applicability was confirmed by detecting SARS-CoV-2 in nasopharyngeal samples from COVID-19 patients without the need of any amplification process.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Nanoestruturas Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Nanoestruturas Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article