Protocol for the production and purification of an i-Motif-specific nanobody.
STAR Protoc
; 4(4): 102729, 2023 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-37995194
ABSTRACT
Intercalated motifs or i-Motifs (iMs) are nucleic acid structures formed by cytosine-rich sequences, which may regulate cellular processes and have broad applications in nanotechnology due to their pH-dependent nature. We have developed an iM-specific nanobody (iMbody) that can recognize iM DNA structures regardless of their sequences, making it a versatile research tool for studying iMs in various contexts. Here, we provide a protocol for the bacterial expression and His-tag purification of iMbody. We then describe procedures for performing ELISA and immunostaining using iMbody.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Nanotecnologia
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article