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Live Imaging of 3D Hanging Drop Arrays through Manipulation of Light-Responsive Pyroelectric Slippery Surface and Chip Adhesion.
Zhou, Shuneng; Yang, Junfeng; Li, Rui; Chen, Yiyu; Li, Chengpan; Chen, Chao; Tao, Yuan; Fan, Shengying; Wu, Dong; Wen, Li; Qiu, Bensheng; Ding, Weiping.
Afiliação
  • Zhou S; Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei, Anhui 230027, China.
  • Yang J; Department of Oncology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China.
  • Li R; Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230027, China.
  • Chen Y; Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230027, China.
  • Li C; Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230027, China.
  • Chen C; Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei, Anhui 230027, China.
  • Tao Y; Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230027, China.
  • Fan S; Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230027, China.
  • Wu D; Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230027, China.
  • Wen L; Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230027, China.
  • Qiu B; Department of Precision Machinery and Instrumentation, University of Science and Technology of China, Hefei 230027, China.
  • Ding W; Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei, Anhui 230027, China.
Nano Lett ; 23(23): 10710-10718, 2023 Dec 13.
Article em En | MEDLINE | ID: mdl-38010943
ABSTRACT
Three-dimensional (3D) hanging drop cell culture is widely used in organoid culture because of its lack of selection pressure and rapid cell aggregation. However, current hanging drop technology has limitations, such as a dependence on complex microfluidic transport channels or specific capillary force templates for drop formation, which leads to unchangeable drop features. These methods also hinder live imaging because of space and complexity constraints. Here, we have developed a hanging drop construction method and created a flexible 3D hanging drop construction platform composed of a manipulation module and an adhesion module. Their harmonious operation allows for the easy construction of hanging drops of varying sizes, types, and patterns. Our platform produces a cell hanging drop chip with small sizes and clear fields of view, thereby making it compatible with live imaging. This platform has great potential for personalized medicine, cancer and drug discovery, tissue engineering, and stem cell research.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Células / Microfluídica Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Células / Microfluídica Idioma: En Ano de publicação: 2023 Tipo de documento: Article