Polyamines permit the preparation of stable Physarum core particles which have a structure similar to those from higher eukaryotes.
Biochim Biophys Acta
; 908(1): 34-45, 1987 Jan 28.
Article
em En
| MEDLINE
| ID: mdl-3801484
ABSTRACT
The inherent instability of Physarum nucleosome core particles prepared by micrococcal nuclease digestion in Na+/Ca2+ buffers can be overcome by the addition of 0.15 mM spermine and 0.5 mM spermidine. Neutron scattering, circular dichroism, nuclease digestion and thermal denaturation studies carried out on these stable monosomes show them to be very similar to those obtained from higher eukaryotes.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Physarum
/
Cromatina
/
Nucleossomos
Limite:
Animals
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article