Efficient Expression in the Prokaryotic Host System, Purification and Structural Analyses of the Recombinant Human ACE2 Catalytic Subunit as a Hybrid Protein with the B Subunit of Cholera Toxin (CTB-ACE2).

Ghahramani, Maryam; Shahsavani, Mohammad Bagher; Khaleghinejad, Seyed Hossein; Niazi, Ali; Moosavi-Movahedi, Ali Akbar; Yousefi, Reza

Ghahramani, Maryam; Shahsavani, Mohammad Bagher; Khaleghinejad, Seyed Hossein; Niazi, Ali; Moosavi-Movahedi, Ali Akbar; Yousefi, Reza.

Afiliação

Ghahramani M; Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
Shahsavani MB; Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
Khaleghinejad SH; Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
Niazi A; Institute of Biotechnology, Shiraz University, Shiraz, Iran.
Moosavi-Movahedi AA; Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
Yousefi R; Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran. ryousefi@shirazu.ac.ir.

Protein J ; 43(1): 24-38, 2024 Feb.

Article em En | MEDLINE | ID: mdl-38017315

Assuntos

Diabetes Mellitus; Hipertensão; Humanos; Toxina da Cólera/genética; Toxina da Cólera/metabolismo; Enzima de Conversão de Angiotensina 2/genética; Enzima de Conversão de Angiotensina 2/metabolismo; Domínio Catalítico

Palavras-chave

Cholera toxin B subunit; Human ACE2 enzyme; Optimizing expression; Purification

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diabetes Mellitus / Hipertensão Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

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