Your browser doesn't support javascript.
loading
Single-cell insights into epithelial morphogenesis in the neonatal mouse uterus.
Spencer, Thomas E; Lowke, Makenzie T; Davenport, Kimberly M; Dhakal, Pramod; Kelleher, Andrew M.
Afiliação
  • Spencer TE; Division of Animal Sciences, University of Missouri, Columbia, MO 65211.
  • Lowke MT; Division of Obstetrics, Gynecology, and Women's Health, University of Missouri, Columbia, MO 65211.
  • Davenport KM; Division of Animal Sciences, University of Missouri, Columbia, MO 65211.
  • Dhakal P; Division of Animal Sciences, University of Missouri, Columbia, MO 65211.
  • Kelleher AM; Division of Animal Sciences, University of Missouri, Columbia, MO 65211.
Proc Natl Acad Sci U S A ; 120(49): e2316410120, 2023 Dec 05.
Article em En | MEDLINE | ID: mdl-38019863
The uterus is vital for successful reproduction in mammals, and two different types of epithelia (luminal and glandular) are essential for embryo implantation and pregnancy establishment. However, the essential cellular and molecular factors and pathways governing postnatal epithelium maturation, determination, and differentiation in developing uterus are yet to be elucidated. Here, the epithelium of the neonatal mouse uterus was isolated and subjected to single-cell transcriptome (scRNA-seq) analysis. Both the undifferentiated epithelium and determined luminal epithelium were heterogeneous and contained several different cell clusters based on single-cell transcription profiles. Substantial gene expression differences were evident as the epithelium matured and differentiated between postnatal days 1 to 15. Two new glandular epithelium-expressed genes (Gas6 and Cited4) were identified and validated by in situ hybridization. Trajectory analyses provided a framework for understanding epithelium maturation, lineage bifurcation, and differentiation. A candidate set of transcription factors and gene regulatory networks were identified that potentially direct epithelium lineage specification and morphogenesis. This atlas provides a foundation important to discover intrinsic cellular and molecular mechanisms directing uterine epithelium morphogenesis during a critical window of postnatal development.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Útero Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Útero Limite: Animals / Pregnancy Idioma: En Ano de publicação: 2023 Tipo de documento: Article