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B cell immune profiles in dysbiotic vermiform appendixes of pancreatic cancer patients.
Vietsch, Eveline E; Latifi, Diba; Verheij, Maaike; van der Oost, Elise W A; de Wilde, Roeland F; Haen, Roel; van den Boom, Anne Loes; Koerkamp, Bas Groot; Doornebosch, Pascal G; van Verschuer, Victorien M T; Ooms, Ariadne H A G; Mohammad, Farzana; Willemsen, Marcella; Aerts, Joachim G J V; Krog, Ricki T; de Miranda, Noel F C C; van den Bosch, Thierry P P; Mueller, Yvonne M; Katsikis, Peter D; van Eijck, Casper H J.
Afiliação
  • Vietsch EE; Department of Pulmonary Medicine, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • Latifi D; Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • Verheij M; Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • van der Oost EWA; Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • de Wilde RF; Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • Haen R; Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • van den Boom AL; Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • Koerkamp BG; Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • Doornebosch PG; Department of Surgery, Reinier de Graaf Hospital, Delft, Netherlands.
  • van Verschuer VMT; Department of Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • Ooms AHAG; Department of Surgery, IJsselland Hospital, Capelle aan den IJssel, Netherlands.
  • Mohammad F; Department of Surgery, IJsselland Hospital, Capelle aan den IJssel, Netherlands.
  • Willemsen M; Department of Pathology, Pathan BV, Rotterdam, Netherlands.
  • Aerts JGJV; Department of Pathology, Erasmus University Medical Center, Rotterdam, Netherlands.
  • Krog RT; Department of Pulmonary Medicine, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • de Miranda NFCC; Department of Pulmonary Medicine, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • van den Bosch TPP; Department of Pulmonary Medicine, Erasmus MC Cancer Institute, Rotterdam, Netherlands.
  • Mueller YM; Department of Pathology, Leiden University Medical Center, Leiden, Netherlands.
  • Katsikis PD; Department of Pathology, Leiden University Medical Center, Leiden, Netherlands.
  • van Eijck CHJ; Department of Pathology, Erasmus University Medical Center, Rotterdam, Netherlands.
Front Immunol ; 14: 1230306, 2023.
Article em En | MEDLINE | ID: mdl-38022530
Pancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest solid tumors and is resistant to immunotherapy. B cells play an essential role in PDAC progression and immune responses, both locally and systemically. Moreover, increasing evidence suggests that microbial compositions inside the tumor, as well as in the oral cavity and the gut, are important factors in shaping the PDAC immune landscape. However, the gut-associated lymphoid tissue (GALT) has not previously been explored in PDAC patients. In this study, we analyzed healthy vermiform appendix (VA) from 20 patients with PDAC and 32 patients with colon diseases by gene expression immune profiling, flow cytometry analysis, and microbiome sequencing. We show that the VA GALT of PDAC patients exhibits markers of increased inflammation and cytotoxic cell activity. In contrast, B cell function is decreased in PDAC VA GALT based on gene expression profiling; B cells express significantly fewer MHC class II surface receptors, whereas plasma cells express the immune checkpoint molecule HLA-G. Additionally, the vermiform appendix microbiome of PDAC patients is enriched with Klebsiella pneumoniae, Bifidobacterium animalis, and Adlercreutzia equolifaciens, while certain commensals are depleted. Our findings may suggest impaired B cell function within the GALT of PDAC patients, which could potentially be linked to microbial dysbiosis. Additional investigations are imperative to validate our observations and explore these potential targets of future therapies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Apêndice / Carcinoma Ductal Pancreático Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Apêndice / Carcinoma Ductal Pancreático Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article