De novo Semi-Synthetic Platform for Monitoring Protein degradation in Live Cells.
Chembiochem
; 25(3): e202300731, 2024 02 01.
Article
em En
| MEDLINE
| ID: mdl-38031893
ABSTRACT
We designed a platform for monitoring the degradation of exogenous proteins in live cells. We engineered a semi-synthetic platform, which consists of Enhanced Green Fluorescent Protein tagged with SpyCatcher to enable its conjugation to a SpyTag peptide bearing a Von Hippel-Lindau E3 ligand, which was delivered to live cells to promote its degradation. This platform lays the ground for studying the degradation of endogenous proteins equipped with SpyTag and for tracking the degradation of post-translationally modified proteins in live cells.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteólise
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article