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RSV neutralizing antibodies in dried blood.
Terstappen, Jonne; Delemarre, Eveline M; Versnel, Anouk; White, Joleen T; Derrien-Colemyn, Alexandrine; Ruckwardt, Tracy J; Bont, Louis J; Mazur, Natalie I.
Afiliação
  • Terstappen J; Department of Pediatric Infectious Diseases and Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands.
  • Delemarre EM; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands.
  • Versnel A; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands.
  • White JT; Center for Translational Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands.
  • Derrien-Colemyn A; Bill & Melinda Gates Medical Research Institute, Cambridge, Massachusetts, United States of America.
  • Ruckwardt TJ; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States of America.
  • Bont LJ; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States of America.
  • Mazur NI; Department of Pediatric Infectious Diseases and Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands.
J Infect Dis ; 2023 Nov 30.
Article em En | MEDLINE | ID: mdl-38035790
BACKGROUND: The key correlate of protection of respiratory syncytial virus (RSV) vaccines and monoclonal antibodies (mAb) is virus neutralization, measured using sera obtained through venipuncture. Dried blood obtained with a finger prick can simplify acquisition, processing, storage, and transport in trials, and thereby reduce costs. In this study we validate an assay to measure RSV neutralization in dried capillary blood. METHODS: Functional antibodies were compared between matched serum and dried blood samples from a phase I trial with RSM01, an investigational anti-RSV Prefusion F mAb. Hep-2 cells were infected with a serial dilution of sample-virus mixture using RSV-A2-mKate to determine half-maximal inhibitory concentration. Stability of dried blood was evaluated over time and during temperature stress. RESULTS: Functional antibodies in dried blood were highly correlated with serum (R2 = 0.98, p < 0.0001). The precision of the assay for dried blood was similar to serum. The function of mAb remained stable for 9 months at room temperature and frozen dried blood samples. INTERPRETATION: We demonstrated the feasibility of measuring RSV neutralization using dried blood as a patient-centered solution that may replace serology testing in trials against RSV or other viruses, such as influenza and SARS-CoV-2.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article