Cryosectioning and Immunohistochemistry Using Frozen Adult Murine Brain Neural Tissue.
Methods Mol Biol
; 2746: 155-163, 2024.
Article
em En
| MEDLINE
| ID: mdl-38070088
ABSTRACT
Cryopreservation and immunohistochemistry offer a comprehensive, robust, and simple methodology to investigate neural patterning and cellular function. Rapid freezing of the whole brain allows excellent preservation of neural ultrastructure and tissue architecture without destroying sensitive protein epitopes that are often compromised following standard paraffin embedding histological techniques. Here, we present a rapid and simple protocol for employing cryosectioning and subsequent immunohistochemistry in the study of adult murine brain neural tissue.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Crioultramicrotomia
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article