Modulation of the capillary leakage by exogenous albumin in a rat model of endothelial glycocalyx damage.
Clin Hemorheol Microcirc
; 2023 Dec 07.
Article
em En
| MEDLINE
| ID: mdl-38073383
ABSTRACT
BACKGROUND:
Endothelial glycocalyx (EG) plays a crucial role in maintaining the plasma proteins within the intravascular space.OBJECTIVE:
We studied whether exogenous albumin protects the EG in an experimental model of EG enzymatic damage in rats.METHODS:
Rats were divided into three groups of 10 animals that received (1) Evans blue (2) Evans blueâ+âhyaluronidase, or (3) Evans blueâ+âhyaluronidaseâ+â20% human albumin via the tail vein. Spectrophotometric analysis was performed 2âh later to quantify the leakage of Evans blue-labeled albumin into the heart, lungs, brain, kidneys, liver, small intestine, spleen, and skeletal muscle.RESULTS:
Administration of hyaluronidase numerically increased the capillary leakage of Evans blue in all examined tissues. Co-administration of albumin decreased the leakage of albumin in all tissues except the heart. In the lungs, the ratio between the absorbance and dry organ weight decreased from 5.3 ± 2.4 to 1.7 ± 0.5 (mean ± SD) (Pâ< â0.002), and in the liver, the absorbance decreased from 2.2 ± 0.7 to 1.5 ± 0.4 (Pâ< â0.011).CONCLUSION:
Exogenous albumin decreased the capillary leakage of albumin which was interpreted as a sign of maintained EG integrity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article