Simultaneous speciation analysis of arsenic and iodine in human urine by high performance liquid chromatography-inductively coupled plasma mass spectrometry.
Anal Sci
; 40(3): 555-562, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38091252
ABSTRACT
A high-performance liquid chromatography-inductively coupled plasma mass spectrometry-based method was developed for the simultaneous determination of four iodine species (i.e. iodate, 3-iodo-tyrosine, 3,5-diiodo-tyrosine, and iodide) and six arsenic species (i.e. arsenobetaine, arsenite, dimethylarsinic acid, arsenocholine, methylarsonic acid, and arsenate) in human urine. The chromatographic separation was performed on a Dionex IonPac As7 anion exchange column. The mobile phase was initiated with 0.5 mmol/L ammonium carbonate solution, followed by 50 mmol/L ammonium carbonate/100 mmol/L ammonium nitrate solution (with 4% methanol). The limits of quantification of the analytes ranged from 0.045 to 2.26 µg/L. At three spiked levels (10.0, 20.0, 50.0 µg/L), the average recoveries (%) ranged from 87.4 to 113.1%, and the relative standard deviations (RSD, %) ranged from 0.4 to 17.2%. The ratio of the sum of six arsenic species to the total arsenic measured by ICPMS ranged from 77.4 to 121.2%, and the ratio of the sum of the four iodine species to the total iodine ranged from 70.7 to 114.7%, indicating a good agreement between these two methods for both arsenic and iodine.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arsênio
/
Carbonatos
/
Iodo
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article