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Bioencapsulation of Oocytes and Granulosa Cells.
Faustini, Massimo; Agradi, Stella; Vigo, Daniele; Torre, Maria L; Curone, Giulio.
Afiliação
  • Faustini M; Department of Veterinary Medicine and Animal Sciences, University of Milan, Milan, Italy.
  • Agradi S; Department of Veterinary Medicine and Animal Sciences, University of Milan, Milan, Italy.
  • Vigo D; Department of Veterinary Medicine and Animal Sciences, University of Milan, Milan, Italy. Daniele.vigo@unimi.it.
  • Torre ML; Dipartimento di Scienze del farmaco, Università degli Studi del Piemonte Orientale, Vercelli, Italy.
  • Curone G; Department of Veterinary Medicine and Animal Sciences, University of Milan, Milan, Italy.
Methods Mol Biol ; 2749: 103-108, 2024.
Article em En | MEDLINE | ID: mdl-38133778
ABSTRACT
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and coculture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a three-dimensional fashion.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Células da Granulosa Limite: Female / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Células da Granulosa Limite: Female / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article