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Base flipping mechanism and binding strength of methyl-damaged DNA during the interaction with AGT.
Koirala, Rajendra Prasad; Adhikari, Narayan Prasad.
Afiliação
  • Koirala RP; Central Department of Physics, Tribhuvan University, Kathmandu, Nepal.
  • Adhikari NP; Central Department of Physics, Tribhuvan University, Kathmandu, Nepal. narayan.adhikari@cdp.tu.edu.np.
J Biol Phys ; 50(1): 71-87, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38150168
ABSTRACT
Methyl damage to DNA bases is common in the cell nucleus. O6-alkylguanine-DNA alkyl transferase (AGT) may be a promising candidate for direct damage reversal in methylated DNA (mDNA) at the O6 point of the guanine. Indeed, atomic-level investigations in the contact region of AGT-DNA complex can provide an in-depth understanding of their binding mechanism, allowing to evaluate the silico-drug nature of AGT and its utility in removing methyl damage in DNA. In this study, molecular dynamics (MD) simulation was utilized to examine the flipping of methylated nucleotide, the binding mechanism between mDNA and AGT, and the comparison of binding strength prior and post methyl transfer to AGT. The study reveals that methylation at the O6 atom of guanine weakens the hydrogen bond (H-bond) between guanine and cytosine, permitting for the flipping of such nucleotide. The formation of a H-bond between the base pair of methylated nucleotide (i.e., cytosine) and the intercalated arginine of AGT also forces the nucleotide to rotate. Following that, electrostatics and van der Waals contacts as well as hydrogen bonding contribute to form the complex of DNA and protein. The stronger binding of AGT with DNA before methyl transfer creates the suitable condition to transfer methyl adduct from DNA to AGT.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: O(6)-Metilguanina-DNA Metiltransferase / Reparo do DNA Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: O(6)-Metilguanina-DNA Metiltransferase / Reparo do DNA Idioma: En Ano de publicação: 2024 Tipo de documento: Article