The utility of smartphone-based quantitative analysis of SARS-CoV-2-specific antibody lateral flow assays.
Diagn Microbiol Infect Dis
; 108(3): 116166, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38157638
ABSTRACT
OBJECTIVES:
Although antibody measurements using lateral flow assay (LFA) kits are convenient, they usually require a specialized reader for quantification. However, a smartphone-based quantification application can be used as a reader for LFA kits. We investigated the quantification ability of the application for SARS-CoV-2-specific antibodies.METHODS:
Eight hundred frozen serum samples from 100 healthcare professionals who received a COVID-19 vaccine were analyzed. Images of assayed LFA kits were obtained using a smartphone camera. We determined whether the ratio of color density of the test and control lines of spike protein IgG correlated with chemiluminescent immunoassay-measured titers.RESULTS:
Spike protein IgG correlated well with the quantification results of the LFA kits using the application installed on a smartphone (r = 0.886).CONCLUSION:
Our results suggest that smartphone-based quantitative analysis of LFA kits enables the quantification of anti-SARS-CoV-2 IgG without special devices, enabling point-of-care assessment of acquired humoral immunity in various settings.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Smartphone
/
COVID-19
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article