Prolonged exposure to lactate causes TREK1 channel clustering in rat hippocampal astrocytes.
Neurosci Lett
; 821: 137613, 2024 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-38157928
ABSTRACT
Increased concentrations of lactate (15-30 mM) are associated with and found to be neuroprotective in various brain pathophysiology. In our earlier studies we showed that high levels of lactate can increase TREK1 channel activity and expression within 1 h. TREK1 channels are two pore domain leak potassium ion channels that are upregulated during cerebral ischemia, epilepsy and other brain pathologies. They play a prominent neuroprotective role against excitotoxicity. Although it has been previously shown that chronic application of lactate (6 h) causes increased gene transcription and protein expression, we observe clustering of TREK1 channels that is dependent on time of exposure (3-6 h) and concentration of lactate (15-30 mM). Using immunofluorescence techniques and image analysis, we show that the clustering of TREK1 channels is dependent on the actin cytoskeletal network of the astrocytes. Clustering of TREK1 channels can augment astrocytic functions during pathophysiological conditions and have significant implications in lactate mediated neuroprotection.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Astrócitos
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Fármacos Neuroprotetores
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Ácido Láctico
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Canais de Potássio de Domínios Poros em Tandem
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Hipocampo
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article