microRNA-622 upregulates cell cycle process by targeting FOLR2 to promote CRC proliferation.
BMC Cancer
; 24(1): 26, 2024 Jan 02.
Article
em En
| MEDLINE
| ID: mdl-38166756
ABSTRACT
BACKGROUND:
Epigenetic alterations contribute greatly to the development and progression of colorectal cancer, and effect of aberrant miR-622 expression is still controversial. This study aimed to discover miR-622 regulation in CRC proliferation.METHODS:
miR-622 expression and prognosis were analyzed in clinical CRC samples from Nanfang Hospital. miR-622 regulation on cell cycle and tumor proliferation was discovered, and FOLR2 was screened as functional target of miR-622 using bioinformatics analysis, which was validated via dual luciferase assay and gain-of-function and loss-of-function experiments both in vitro and in vivo.RESULTS:
miR-622 overexpression in CRC indicated unfavorable prognosis and it regulated cell cycle to promote tumor growth both in vitro and in vivo. FOLR2 is a specific, functional target of miR-622, which negatively correlates with signature genes in cell cycle process to promote CRC proliferation.CONCLUSIONS:
miR-622 upregulates cell cycle process by targeting FOLR2 to promote CRC proliferation, proposing a novel mechanism and treatment target in CRC epigenetic regulation of miR-622.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
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MicroRNAs
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Proliferação de Células
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Receptor 2 de Folato
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article