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Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system.
Yi, Yating; Li, Youqi; Zhang, Shiwen; Men, Yi; Wang, Yuhong; Jing, Dian; Ding, Jiayi; Zhu, Qingjie; Chen, Zexi; Chen, Xingjun; Li, Jun-Liszt; Wang, Yilong; Wang, Jun; Peng, Hanchuan; Zhang, Li; Luo, Wenjing; Feng, Jian Q; He, Yongwen; Ge, Woo-Ping; Zhao, Hu.
Afiliação
  • Yi Y; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
  • Li Y; Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
  • Zhang S; Chinese Institute for Brain Research, Beijing, China.
  • Men Y; Chinese Institute for Brain Research, Beijing, China.
  • Wang Y; Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
  • Jing D; School of Basic Medical Sciences, Capital Medical University, Beijing, China.
  • Ding J; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
  • Zhu Q; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
  • Chen Z; State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
  • Chen X; Department of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
  • Li JL; West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan, China.
  • Wang Y; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shangha
  • Wang J; Chinese Institute for Brain Research, Beijing, China.
  • Peng H; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
  • Zhang L; Chinese Institute for Brain Research, Beijing, China.
  • Luo W; Chinese Institute for Brain Research, Beijing, China.
  • Feng JQ; Chinese Institute for Brain Research, Beijing, China.
  • He Y; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
  • Ge WP; Chinese Institute for Brain Research, Beijing, China.
  • Zhao H; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
Cell Res ; 34(2): 124-139, 2024 02.
Article em En | MEDLINE | ID: mdl-38168640
ABSTRACT
Achieving uniform optical resolution for a large tissue sample is a major challenge for deep imaging. For conventional tissue clearing methods, loss of resolution and quality in deep regions is inevitable due to limited transparency. Here we describe the Transparent Embedding Solvent System (TESOS) method, which combines tissue clearing, transparent embedding, sectioning and block-face imaging. We used TESOS to acquire volumetric images of uniform resolution for an adult mouse whole-body sample. The TESOS method is highly versatile and can be combined with different microscopy systems to achieve uniformly high resolution. With a light sheet microscope, we imaged the whole body of an adult mouse, including skin, at a uniform 0.8 × 0.8 × 3.5 µm3 voxel resolution within 120 h. With a confocal microscope and a 40×/1.3 numerical aperture objective, we achieved a uniform sub-micron resolution in the whole sample to reveal a complete projection of individual nerve axons within the central or peripheral nervous system. Furthermore, TESOS allowed the first mesoscale connectome mapping of individual sensory neuron axons spanning 5 cm from adult mouse digits to the spinal cord at a uniform sub-micron resolution.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Axônios / Imageamento Tridimensional Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Axônios / Imageamento Tridimensional Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article