Determination for KIR genotype and allele copy number via real-time quantitative PCR method.
Immunogenetics
; 76(2): 137-143, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38206349
ABSTRACT
Killer cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) play crucial roles in regulating NK cell activity. Here, we report a real-time quantitative PCR (qPCR) to genotype all KIR genes and their copy numbers simultaneously. With 18 pairs of locus-specific primers, we identified KIR genes by Ct values and determined KIR copy number using the 2-∆Ct method. Haplotypes were assigned based on KIR gene copy numbers. The real-time qPCR results were consistent with the NGS method, except for one sample with KIR2DL5 discrepancy. qPCR is a multiplex method that can identify KIR copy number, which helps obtain a relatively accurate haplotype structure, facilitating increased KIR research in laboratories where NGS or other high-resolution methods are not available.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores KIR
/
Variações do Número de Cópias de DNA
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article