Molecular monitoring of treatment efficacy in human visceral leishmaniasis.
Trans R Soc Trop Med Hyg
; 118(5): 343-345, 2024 May 01.
Article
em En
| MEDLINE
| ID: mdl-38223920
ABSTRACT
BACKGROUND:
Focused efforts of the visceral leishmaniasis elimination program have led to a drastic decline in cases, and the present challenge is disease monitoring, which this study aimed to assess.METHODS:
A Leishmania kinetoplastid-targeted qPCR quantified parasite load at disease presentation, and following treatment completion (n=49); an additional 80 cases were monitored after completion of treatment.RESULTS:
The parasite load at disease presentation was 13 461.00 (2560.00-37764.00)/µg gDNA, which upon completion of treatment reduced in 47 of 49 cases to 1(1-1)/µg gDNA, p<0.0001. In 80 cases that presented >2 months post-treatment, their parasite burden similarly decreased to 1(1-1)/µg gDNA except in 6 of 80 cases, which were qPCR positive.CONCLUSION:
In 129 cases of visceral leishmaniasis, qPCR by quantification of parasite burden proved effective for monitoring treatment.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carga Parasitária
/
Reação em Cadeia da Polimerase em Tempo Real
/
Leishmaniose Visceral
/
Antiprotozoários
Limite:
Adolescent
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Adult
/
Aged
/
Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article