Expression of active caspase 3 in the rat lens after in vivo exposure to subthreshold dose of UVR-B.
BMC Ophthalmol
; 24(1): 29, 2024 Jan 22.
Article
em En
| MEDLINE
| ID: mdl-38254051
ABSTRACT
PURPOSES:
The aim of this study is to investigate the time evolution of active caspase 3 within first 120 h in the rat lens after in vivo exposure to subthreshold dose of UVR-B.METHODS:
Twenty three six-week-old female albino Sprague-Dawley rats were exposed to subthreshold dose (1 kJ/m2) of UVR-B unilaterally and sacrificed at 24, 41, 70 and 120 h after exposure. Lenses were enucleated and active caspase 3 was detected by Western Blot. The time evolution of active caspase 3 was then plotted as a function of relative mean difference in active caspase 3 between exposed and nonexposed lenses.RESULTS:
There is expression of active caspase 3 in both exposed and nonexposed lenses but there is no difference in relative mean difference in active caspase 3 between exposed and nonexposed lenses in all four postexposure groups.CONCLUSIONS:
Exposure to subthreshold dose of UVR-B does not induce apoptosis in the rat lens in vivo within first 120 h though there is a non-significant increase of active caspase 3 at 120 h. Increase in sample size might reduce the variation level in expression of active caspase 3 in the rat lenses.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
/
Caspase 3
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Cristalino
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article