Optimizing enzyme-responsive polymersomes for protein-based therapies.
Nanomedicine (Lond)
; 19(3): 213-229, 2024 02.
Article
em En
| MEDLINE
| ID: mdl-38271081
ABSTRACT
Aims:
Stimuli-responsive polymersomes are promising tools for protein-based therapies, but require deeper understanding and optimization of their pathology-responsive behavior. Materials &methods:
Hyaluronic acid (HA)-poly(b-lactic acid) (PLA) polymersomes self-assembled from block copolymers of varying molecular weights of HA were compared for their physical properties, degradation and intracellular behavior.Results:
Major results showed increasing enzyme-responsivity associated with decreasing molecular weight. The major formulation differences were as follows the HA(5 kDa)-PLA formulation exhibited the most pronounced release of encapsulated proteins, while the HA(7 kDa)-PLA formulation showed the most different release behavior from neutral.Conclusion:
We have discovered design rules for HA-PLA polymersomes for protein delivery, with lower molecular weight leading to higher encapsulation efficiency, greater release and greater intracellular uptake.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
/
Polímeros
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article