Protocol for acquiring high-quality fresh mouse lung spatial transcriptomics data.
STAR Protoc
; 5(1): 102825, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38280199
ABSTRACT
Spatial transcriptomics analysis allows the examination of the biological characteristics and spatial distribution of individual lung cells at a single-cell resolution. However, due to the presence of cavities in the alveoli of the lungs, it is challenging to section them for spatial transcriptomics experiments. Here, we present a protocol for acquiring high-quality fresh mouse lung spatial transcriptomics data. We describe steps for lung perfusion, acquiring frozen slices, collecting cDNA from lung sections, and data analysis. For complete details on the use and execution of this protocol, please refer to Jiang et al.1.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Perfilação da Expressão Gênica
/
Análise de Dados
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article