Sensitive and selective electrochemical aptasensing method for the voltammetric determination of dopamine based on AuNPs/PEDOT-ERGO nanocomposites.
Bioelectrochemistry
; 157: 108653, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38281365
ABSTRACT
In this study, the effects of phosphate buffered saline (PBS) and graphene oxide (GO) as supporting electrolytes and dopants on the electropolymerization process of 3,4-ethylenedioxythiophene (EDOT) on glassy carbon electrode (GCE) were investigated. It was found that the PEDOT-ERGO nanocomposites obtained by a simple one-step electrochemical redox polymerization method using GO as the only supporting electrolyte and dopant possess excellent electrochemical properties. Then, the PEDOT-ERGO nanocomposites were used as electrode substrate to further modify with AuNPs, and an electrochemical aptasensor based on AuNPs/PEDOT-ERGO nanocomposites was successfully constructed for the sensitive and selective determination of dopamine (DA). Comparison of the cyclic voltammetric response of different neurotransmitters before and after aptamer assembly showed that the aptamer significantly improved the selectivity of the sensor for DA. The low detection limit of 1.0 µM (S/N = 3) indicated the good electrochemical performance of the PEDOT-ERGO nanocomposite film. Moreover, the aptasensor showed good recoveries in 50-fold diluted fetal bovine serum with RSD values all less than 5 % (n = 5), indicating that the PEDOT-ERGO nanocomposites and the electrochemical aptasensor have promising applications in other neurochemicals assay and biomedical analysis.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nanocompostos
/
Nanopartículas Metálicas
/
Grafite
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article