Biochemical Markers for Liver Injury in Zebrafish Larvae.
Methods Mol Biol
; 2753: 469-482, 2024.
Article
em En
| MEDLINE
| ID: mdl-38285360
ABSTRACT
Liver plays a crucial role in detoxification processes and metabolism of xenobiotics, and therefore, it is a target organ of toxicity of different classes of chemicals. In this context, some key enzymes present in liver are considered to be good biochemical markers of hepatic damage and can have their activities determined via spectrophotometry. Aspartate and alanine aminotransferases, alkaline phosphatase, lactate dehydrogenase, and glutathione peroxidase are enzymes that have activities often changed in response to hepatotoxic compounds and can be accessed through the larval period of zebrafish (Danio rerio). In this chapter, we described methodologies for analyses of these five biomarkers in pooled zebrafish larvae through spectrophotometry.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Perciformes
/
Peixe-Zebra
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article