Deciphering the crystal structure of a novel nanobody against the NEIL1 DNA glycosylase.
Acta Crystallogr D Struct Biol
; 80(Pt 2): 137-146, 2024 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-38289715
ABSTRACT
Nanobodies (VHHs) are single-domain antibodies with three antigenic CDR regions and are used in diverse scientific applications. Here, an â¼14â
kDa nanobody (A5) specific for the endonuclease VIII (Nei)-like 1 or NEIL1 DNA glycosylase involved in the first step of the base-excision repair pathway was crystallized and its structure was determined to 2.1â
Å resolution. The crystals posed challenges due to potential twinning and anisotropic diffraction. Despite inconclusive twinning indicators, reprocessing in an orthorhombic setting and molecular replacement in space group P21212 enabled the successful modeling of 96% of residues in the asymmetric unit, with final Rwork and Rfree values of 0.199 and 0.229, respectively.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Glicosilases
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article