Production and Purification of Antibodies in Chinese Hamster Ovary Cells.
Methods Mol Biol
; 2762: 183-190, 2024.
Article
em En
| MEDLINE
| ID: mdl-38315366
ABSTRACT
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of monoclonal IgG antibodies from Chinese hamster ovary cells. It includes steps for cell maintenance, transient transfection, and antibody purification via protein A affinity chromatography. The methods described are intended for the production of milligram amounts of protein but can be adapted for most small- to mid-scale applications.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article