Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules.
Nat Methods
; 21(3): 401-405, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38317008
ABSTRACT
Unique molecular identifiers are random oligonucleotide sequences that remove PCR amplification biases. However, the impact that PCR associated sequencing errors have on the accuracy of generating absolute counts of RNA molecules is underappreciated. We show that PCR errors are a source of inaccuracy in both bulk and single-cell sequencing data, and synthesizing unique molecular identifiers using homotrimeric nucleotide blocks provides an error-correcting solution that allows absolute counting of sequenced molecules.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sequenciamento de Nucleotídeos em Larga Escala
/
Nucleotídeos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article