Cloning of three epsilon-class glutathione S-transferase genes from Micromelalopha troglodyta (Graeser) (Lepidoptera: Notodontidae) and their response to tannic acid.
Bull Entomol Res
; 114(2): 172-179, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38327098
ABSTRACT
Micromelalopha troglodyta (Graeser) is an important pest of poplar in China, and glutathione S-transferase (GST) is an important detoxifying enzyme in M. troglodyta. In this paper, three full-length GST genes from M. troglodyta were cloned and identified. These GST genes all belonged to the epsilon class (MtGSTe1, MtGSTe2, and MtGSTe3). Furthermore, the expression of these three MtGSTe genes in different tissues, including midguts and fat bodies, and the MtGSTe expression in association with different concentrations of tannic acid, including 0.001, 0.01, 0.1, 1, and 10 mg ml-1, were analysed in detail. The results showed that the expression levels of MtGSTe1, MtGSTe2, and MtGSTe3 were all the highest in the fourth instar larvae; the expression levels of MtGSTe1 and MtGSTe3 were the highest in fat bodies, while the expression level of MtGSTe2 was the highest in midguts. Furthermore, the expression of MtGSTe mRNA was induced by tannic acid in M. troglodyta. These studies were helpful to clarify the interaction between plant secondary substances and herbivorous insects at a deep level and provided a theoretical foundation for controlling M. troglodyta.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Taninos
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Glutationa Transferase
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Mariposas
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article