Dynamic selection of high-affinity aptamers using a magnetically activated continuous deflection microfluidic chip.
Chem Commun (Camb)
; 60(20): 2772-2775, 2024 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-38353965
ABSTRACT
To accelerate the discovery of high-affinity aptamers, a magnetically activated continuous deflection (MACD) chip was designed. The MACD chip could achieve dynamic selection in a continuous flow, which meant that the binding and separation were carried out consecutively. Dynamic selection could make selection efficient. Low-affinity sequences could be eluted in time and high-affinity sequences could be enriched via dynamic selection. The stringency of the conditions could be further increased by lowering the target concentration in the dynamic selection. Finally, a C.al3 aptamer with high-affinity and high-specificity for Candida albicans (C. albicans) was obtained through six rounds of selection. Its dissociation constant (Kd) was 7.9 nM. This demonstrated that dynamic selection using a MACD chip was an effective method for high-affinity aptamer selection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microfluídica
/
Aptâmeros de Nucleotídeos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article