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Formation of an instantaneous nick for highly efficient adenylation of oligonucleotides by ligase without subsequent jointing.
Hu, Kunling; Sun, Wenhua; Chen, Hui; Luo, Jian; Song, Ziting; An, Ran; Komiyama, Makoto; Liang, Xingguo.
Afiliação
  • Hu K; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No. 1299 Sansha Road, Qingdao, P. R. China. ar@ouc.edu.cn.
  • Sun W; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No. 1299 Sansha Road, Qingdao, P. R. China. ar@ouc.edu.cn.
  • Chen H; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No. 1299 Sansha Road, Qingdao, P. R. China. ar@ouc.edu.cn.
  • Luo J; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No. 1299 Sansha Road, Qingdao, P. R. China. ar@ouc.edu.cn.
  • Song Z; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No. 1299 Sansha Road, Qingdao, P. R. China. ar@ouc.edu.cn.
  • An R; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No. 1299 Sansha Road, Qingdao, P. R. China. ar@ouc.edu.cn.
  • Komiyama M; Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao, P. R. China.
  • Liang X; Research Center for Advanced Science and Technology (RCAST), The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan.
Chem Commun (Camb) ; 60(21): 2942-2945, 2024 Mar 07.
Article em En | MEDLINE | ID: mdl-38374791
ABSTRACT
By forming a nick at the adenylation site instantaneously, nucleic acids are efficiently adenylated by T4 DNA ligase. The subsequent ligation is successfully suppressed in terms of rapid conversion of the instantaneous nick to a more stable gap. It is helpful to understand enzymatic ligation dynamics, and the adenylated products can be used for various practical applications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligonucleotídeos / Ligases Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligonucleotídeos / Ligases Idioma: En Ano de publicação: 2024 Tipo de documento: Article