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Aberrant cortex contractions impact mammalian oocyte quality.
Nikalayevich, Elvira; Letort, Gaëlle; de Labbey, Ghislain; Todisco, Elena; Shihabi, Anastasia; Turlier, Hervé; Voituriez, Raphaël; Yahiatene, Mohamed; Pollet-Villard, Xavier; Innocenti, Metello; Schuh, Melina; Terret, Marie-Emilie; Verlhac, Marie-Hélène.
Afiliação
  • Nikalayevich E; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, 75005 Paris, France.
  • Letort G; Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, Université Paris Cité, 25 rue du Dr. Roux, 75015 Paris, France.
  • de Labbey G; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, 75005 Paris, France.
  • Todisco E; Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
  • Shihabi A; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, 75005 Paris, France.
  • Turlier H; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, 75005 Paris, France.
  • Voituriez R; Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), Laboratoire Jean Perrin, CNRS, Sorbonne Université, Paris, France.
  • Yahiatene M; Centre Assistance Médicale à la Procréation Nataliance, Groupe Mlab, Pôle Santé Oréliance, Saran, France.
  • Pollet-Villard X; Centre Assistance Médicale à la Procréation Nataliance, Groupe Mlab, Pôle Santé Oréliance, Saran, France.
  • Innocenti M; Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
  • Schuh M; Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
  • Terret ME; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, 75005 Paris, France. Electronic address: marie-emilie.terret@college-de-france.fr.
  • Verlhac MH; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, 75005 Paris, France. Electronic address: marie-helene.verlhac@college-de-france.fr.
Dev Cell ; 59(7): 841-852.e7, 2024 Apr 08.
Article em En | MEDLINE | ID: mdl-38387459
ABSTRACT
The cortex controls cell shape. In mouse oocytes, the cortex thickens in an Arp2/3-complex-dependent manner, ensuring chromosome positioning and segregation. Surprisingly, we identify that mouse oocytes lacking the Arp2/3 complex undergo cortical actin remodeling upon division, followed by cortical contractions that are unprecedented in mammalian oocytes. Using genetics, imaging, and machine learning, we show that these contractions stir the cytoplasm, resulting in impaired organelle organization and activity. Oocyte capacity to avoid polyspermy is impacted, leading to a reduced female fertility. We could diminish contractions and rescue cytoplasmic anomalies. Similar contractions were observed in human oocytes collected as byproducts during IVF (in vitro fertilization) procedures. These contractions correlate with increased cytoplasmic motion, but not with defects in spindle assembly or aneuploidy in mice or humans. Our study highlights a multiscale effect connecting cortical F-actin, contractions, and cytoplasmic organization and affecting oocyte quality, with implications for female fertility.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Fuso Acromático Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Fuso Acromático Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article